![]() Nelson CM, Tien J (2006) Microstructured extracellular matrices in tissue engineering and development. Maurin B, Canadas P, Baudriller H et al (2008) Mechanical model of cytoskeleton structuration during cell adhesion and spreading. Ki CS, Park SY, Kim HJ et al (2008) Development of 3-D nanofibrous fibroin scaffold with high porosity by electrospinning: implications for bone regeneration. Khang D, Lu J, Yao C et al (2008) The role of nanometer and sub-micron surface features on vascular and bone cell adhesion on titanium. Jou CH, Chen WC, Yang MC et al (2008) In vitro biocompatibility of three-dimensional chitosan scaffolds immobilized with chondroitin-6-sulfate. Hong Z, Zhang P, He C et al (2005) Nano-composite of poly(L-lactide) and surface grafted hydroxyapatite: mechanical properties and biocompatibility. Hay ED (Ed) (1991) Cell biology of extracellular matrix. Diabetologia 40(5):606–609Ĭhua KN, Lim WS, Zhang PC et al (2005) Stable immobilization of rat hepatocyte spheroids on galactosylated nanofiber scaffold. doi: 10.1016/j.msec.2012.04.024.Berti L, Kellerer M, Capp E et al (1997) Leptin stimulates glucose transport and glycogen synthesis in C2C12 myotubes: evidence for a P13-kinase mediated effect. Composite poly-L-lactic acid/poly-(α,β)-DL-aspartic acid/collagen nanofibrous scaffolds for dermal tissue regeneration. Ravichandran R., Venugopal J.R., Sundarrajan S., Mukherjee S., Sridhar R., Ramakrishna S. Electrospun nanofibers for pharmaceutical and medical applications. ![]() Sridhar R., Venugopal J., Sundarrajan S., Ravichandran R., Ramalingam B., Ramakrishna S. Precipitation of nanohydroxyapatite on PLLA/PBLG/Collagen nanofibrous structures for the differentiation of Adipose Derived Stem Cells to Osteogenic lineage. Ravichandran R., Venugopal J.R., Sundarrajan S., Mukherjee S., Ramakrishna S. Fabrication of a nanofibrous scaffold with improved bioactivity for culture of human dermal fibroblasts for skin regeneration. 27–73.Ĭhandrasekaran A.R., Venugopal J., Sundarrajan S., Ramakrishna S. Preparation and properties of nanopolymer advanced composites: A review pp. Polymer-based Nanocomposites for Energy and Environmental Applications. Īnbusagar N., Palanikumar K., Ponshanmugakumar A. Compared to PLLA scaffolds, more mature hepatocyte-like cells with cuboidal-to-polygonal morphology were observed (shown by white arrows) on PLLA/gelatin scaffolds in either induction condition (scale bar = 100 μm.). ( e, j, o) Undifferentiated BMSCs on PLLA/gelatin scaffolds did not express any hepatic markers. Alexa Fluor-594 labelled α-fetoprotein ( a– d: red), Alexa Fluor-488 labelled albumin ( f– i: green) and Alexa Fluor-488 labelled cytokeratin-18 ( k– n: green) expression represents features of hepatocyte-like cells. ![]() Hepatocyte-specific marker expression (α-fetoprotein, albumin and cytokeratin-18) in BMSCs-derived hepatocyte-like cells as shown by confocal microscopy images (merged) on ( a, f, k) PLLA scaffold with recombinant hepatic growth factor induction, ( b, g, l) PLLA scaffold with hepatogenic serum induction, ( c, h, m) PLLA/gelatin with recombinant hepatic growth factor induction, ( d, i, n) PLLA/gelatin scaffold with hepatogenic serum induction at day 28. We have also described novel strategies that include modifications, such as galactosylation, matrix protein incorporation, etc., in the electrospun scaffolds that have evolved to support the long-term growth and viability of the primary hepatocytes.Įlectrospinning extracellular matrix proteins hepatocytes liver tissue engineering nanofibers natural and synthetic polymers. ![]() We have highlighted the use of synthetic and natural electrospun polymers along with liver ECM in the fabrication of these scaffolds. In the current review, we have discussed the various technical aspects of electrospinning that have been employed for scaffold development for different types of liver cells. Electrospinning is one of the most preferred techniques to produce nanofiber scaffolds. Nanofibrous scaffolds have been widely used in the field of tissue engineering for their increased surface-to-volume ratio and increased porosity, and their close resemblance with the native tissue extracellular matrix (ECM) environment. Several strategies have been explored in the recent past for culturing the liver cells in the most apt environment using biological scaffolds supporting hepatocyte growth and differentiation. The major goal of liver tissue engineering is to reproduce the phenotype and functions of liver cells, especially primary hepatocytes ex vivo. ![]()
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